Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L

Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, thepomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-criticalfluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile,carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids(PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source ofγ- tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity usingα-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51–91.54μg/mL), and against normal MR.