SIMToolbox: A MATLAB toolbox for structured illumination fluorescence microscopy
- 1. First Faculty of Medicine, Charles University in Prague, Prague 12800, Czech Republic
- 2. Department of Radioelectronics, Faculty of Electrical Engineering, Czech Technical University in Prague, Prague 16627, Czech Republic; Laboratoire d'Optique Biome´dicale, E´cole Polytechnique Fe´de´rale de Lausanne, Lausanne CH-1015, Switzerland
- 3. Department of Radioelectronics, Faculty of Electrical Engineering, Czech Technical University in Prague, Prague 16627, Czech Republic
- 4. UCCS center for the BioFrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA
Description
SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly graphical interface and designed for processing two-dimensional and three-dimen- sional data acquired by structured illumination microscopy (SIM). Both optical sectioning and super-resolution applications are supported. The software is also capable of maximum a posteriori probability image estimation (MAP-SIM), an alternative method for reconstruction of structured il- lumination images. MAP-SIM can potentially reduce reconstruction artifacts, which commonly occur due to refractive index mismatch within the sample and to imperfections in the illumination.
Files
Actin (green LED) LCOS.zip
Files
(748.7 MB)
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