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Published October 6, 2015 | Version v1
Dataset Open

SIMToolbox: A MATLAB toolbox for structured illumination fluorescence microscopy

  • 1. First Faculty of Medicine, Charles University in Prague, Prague 12800, Czech Republic
  • 2. Department of Radioelectronics, Faculty of Electrical Engineering, Czech Technical University in Prague, Prague 16627, Czech Republic; Laboratoire d'Optique Biome´dicale, E´cole Polytechnique Fe´de´rale de Lausanne, Lausanne CH-1015, Switzerland
  • 3. Department of Radioelectronics, Faculty of Electrical Engineering, Czech Technical University in Prague, Prague 16627, Czech Republic
  • 4. UCCS center for the BioFrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA

Description

SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly graphical interface and designed for processing two-dimensional and three-dimen- sional data acquired by structured illumination microscopy (SIM). Both optical sectioning and super-resolution applications are supported. The software is also capable of maximum a posteriori probability image estimation (MAP-SIM), an alternative method for reconstruction of structured il- lumination images. MAP-SIM can potentially reduce reconstruction artifacts, which commonly occur due to refractive index mismatch within the sample and to imperfections in the illumination.

Files

Actin (green LED) LCOS.zip

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