Published August 13, 2020
| Version 1.0.0
Dataset
Open
Human and mouse ALS tissue sections immunolabeled for FUS, SFPQ, ChAT and counterstained with DAPI'
Authors/Creators
- 1. Francis Crick Institute
- 2. University College London
- 3. Kings College
- 4. Idiap Research Institute
Description
Multichannel microscopy data from histopathological sections of
1/ Tissue sections from spinal cord of SOD1- and VCP-mutant ALS mouse models together with control immunolabeled for FUS, SFPQ, ChAT and counterstained with DAPI.
2/ Tissue sections from spinal cord of Healthy and sporadic ALS donors immunolabeled for FUS or SFPQ, ChAT and counterstained with DAPI.
3/ Scripts to preprocess these images.
These two series of multichannel fluorescent microscopy data have been used in several manuscripts including:
- Luisier R, Tyzack GE, Hall CE, Mitchell JS, Devine H, Taha DM, et al. Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS. Nat Commun 2018; 9: 2010.
- Tyzack GE, Luisier R, Taha DM, Neeves J, Modic M, Mitchell JS, et al. Widespread FUS mislocalization is a molecular hallmark of amyotrophic lateral sclerosis. Brain 2019; 142: 2572–80.