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Published August 12, 2020 | Version v1
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An experimental test of parasite adaptation to common vs. rare host genotypes

Authors/Creators

  • 1. University of Virginia
  • 2. Emory University

Description

A core hypothesis in coevolutionary theory proposes that parasites adapt to specifically infect common host genotypes.  Under this hypothesis, parasites function as agents of negative frequency-dependent selection, favoring rare host genotypes.  This parasite-mediated advantage of rarity is key to the idea that parasites maintain genetic variation and select for outcrossing in host populations.  Here, we report the results of an experimental test of parasite adaptation to common vs. rare host genotypes.  We selected the bacterial parasite Serratia marcescens to kill C. elegans hosts in uneven mixtures of host genotypes.  To examine the effect of commonness itself, independent of host identity, each of four host genotypes was represented as common or rare in experimental host mixtures.  After experimental selection, we evaluated a parasite line's change in virulence, the selected fitness trait, on its rare and common host genotypes.  Our results were consistent with a slight advantage for rare host genotypes: on average, parasites lost virulence against rare genotypes but not against common genotypes.  The response varied substantially, however, with distinct patterns across host genotype mixtures.  These findings support the potential for parasites to impose negative frequency-dependent selection, while emphasizing that the cost of being common may vary with host genotype.

Notes

Data and analysis scripts for Gibson et al. 2020 An experimental test of parasite adaptation to common versus rare host genotypes

Analysis file:

  1. Gibson_BiolLett_Revision.rmd - contains all analyses associated with the paper

Data files:

  1. CommonClone_ancestor: associated with Fig S1; contains ID(unique ID code), Experiment (experimental evolution project, either 1, AKG or 2, PSW), date of mortality assay, assayed host genotype, technical replicate, individual counting bodies, number of live hosts on mortality assay plates, total number added, number estimated to be dead, mortality rate, and average and SE across technical replicates
  2. CommonClone_homogeneous: associated with Fig S2 and Table S1 and text of the Supplemental Results; contains Experiment, Assay (two distinct assays), assay date, experimental treatment of parasite lineage, parasite lineage replicate number, assayed host genotype, technical replicate in mortality assay, individual counting bodies, number of live hosts on mortality assay plates, total number added, number estimated to be dead, and mortality rate
  3. CommonClone_dataset: focal dataset presented in Fig 2, Table S2, Table S3, and text of the Results and Supplemental Results; contains Experimenta, assay, assay date, experimental treatment (heterogeneous host combination in which parasite lineage was selected), parasite lineage replicate number, assayed host genotype, frequency of host genotype during experimental selection, host genotype that was common in experimental selection, technical replicate of mortality assay, individual counting bodies, number of live hosts on mortality assay plates, total number added, number estimated to be dead, mortality rate, mortality rate of ancestral parasites on assayed host genotype and standard error, difference between evolved mortality and ancestral mortality, proportional change from ancestral parasites
  4. CommonClone_meta: dataset for meta-analysis in Fig. S3 and Supplemental Results; derived from CommonClone_dataset, includes calculations of effect sizes

Funding provided by: National Institute of General Medical Sciences
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000057
Award Number: K12GM000680

Funding provided by: National Science Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001
Award Number: DEB-1750553

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Additional details

Related works

Is cited by
10.1111/jeb.13608 (DOI)
10.1098/rsbl.2019.0744 (DOI)