Published August 13, 2020 | Version v1
Dataset Open

Strausberg_Tribolium_LA-GFP_tailpole_run (Excerpt timepoints 291-340)

  • 1. CSBD / MPI CBG

Description

Tribolium castaneum, life-actin GFP (transgenic), imaged in a custom dual-illumination light sheet microscope

This is an excerpt of time points 291-340 showing a mitotic wave.

Voxel size: 0.52 x 0.52 x 2.04 microns 
Frame delay: 1 minute 

Notes (whole data set)

sample author:        Daniela Vorkel

organism:        Tribolium castaneum, life-actin GFP
sample count:        1    
    
fluorescence:         488 (GFP)

frames:            1361
acqu. time:        22h40m
size:            316.8 GB        

sample description:    early embryo, mitotic waves + gastrulation, tailpole view

experimental notes:    optics prefused
 

File descriptions

  • ClearControl meta header files: 
    • C0opticsprefused.index.txt
    • C0opticsprefused.metadata.txt
  • ClearControl program:
    • program.txt
  • Background subtracted / maximum projected thumbnails as video: 
    • Strausberg_thumbnails_sb_text_291-340.gif
  • Singe time point 3D stack:
    • Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-291.tif
  • Image stacks with 10 time points each:
    • Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-291-300.tif
    • Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-301-310.tif
    • Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-311-320.tif
    • Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-321-330.tif
    • Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-331-340.tif

Notes

This is an excerpt of the whole data set showing a single mitotic wave.

Files

C0opticsprefused.index.txt