Published July 23, 2020 | Version v1
Dataset Open

Catalysis of MUF-triNAG by hen egg-white lysozyme (dataset formatted for analysis by interferENZY)

Authors/Creators

  • 1. LEPABE – Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Departamento de Engenharia Química, Faculdade de Engenharia da Universidade do Porto
  • 2. i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto

Description

Main description

This dataset depicts the catalysis of the fluorogenic substrate MUF-triNAG to the fluorescent substrate MUF by hen egg-white lysozyme, for fixed concentration of enzyme and variation of concentration of initial substrate. It was originally documented in Phys. Chem. Chem. Phys., 2020, 22, 16143-16149 (https://doi.org/10.1039/D0CP02469D) and then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis.

 

Dataset organization

Line 1: Tab-separated initial concentrations of substrate MUF-triNAG in micromolar (µM) concentration

Line 2: Concentration of enzyme (0.25 µM)

Line 3: Units of time

Line 4: Units of concentration for substrate values and measurements

Line 5: Dataset name

Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1

 

Contact information:

Maria Filipa Pinto (mfpinto@i3s.up.pt)
Pedro M. Martins (pmartins@ibmc.up.pt)

i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: +351 226 074 900

Notes

Acknowledgements: This work was financed by (i) the European Regional Development Fund (ERDF) through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia (FCT/MCTES) in the framework of grants POCI-01-0145-FEDER-031173, POCI-01-0145-FEDER-007274, POCI-01-0145-FEDER-031323 ("Institute for Research and Innovation in Health Sciences"), (ii) Base funding – UIDB/00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE – funded by national funds through FCT/MCTES (PIDDAC), and by (iii) the ERDF through Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement in the framework of Project Norte-01-0145-FEDER-000008. MFP gratefully acknowledges PhD fellowship SFRH/BD/109324/2015 from FCT, Portugal.

Files

interferENZY_lysozyme.txt

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Additional details

Related works

Is derived from
Journal article: 10.1039/D0CP02469D (DOI)
Is supplement to
Software: 10.5281/zenodo.3956410 (DOI)

References

  • Phys. Chem. Chem. Phys., 2020, 22, 16143-16149