QUALITATIVE ANALYSIS OF EUSIDEROXYLON ZWAGERI TEIJSM AND BINN SEED BY GC-MS AND LC-MS

Background: Eusideroxylonzwageri is an endemic tree in certain areas of Borneo and Sumatra. Limited reports are available for the ethno medical use, phytochemical constituents, pharmacological activities of its seed. Objective: The present study was focused on the phytochemical constituents of E.zwageri seed extracts, polar (aqueous) and nonpolar (Ethanol and Ethyl Acetate) compounds.

Methods: GC-MS and LC-MS techniques were used to establish the phytochemical profiles of the studied extracts.

Result: β-Asarone was detected as single dominant component of the ethanol and ethyl acetate seed extract from E.zwageri. The GC-MS result of the ethanol and ethyl acetate extracts exerted considerable β-Asaron, with 82.44 and 73.05 area % respectively. Additionally, four main flavonoids were found in the aqueous seed extract, namely N-cis-Feruloyltypamine, 3′-O-Methylviolanone and two chromonesN-cis-Feruloyltypaminewas detected mainly in ethanol seed extract, with 148.787 response. 3′-O-Methylviolanone was detected in water and ethyl acetate seed extracts, with 58.178 and 55.730 response respectively. Two chromones were recognized. First, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8-methoxy-chroman-4-one was detected in ethyl acetate extract of the seed with 94.652 response. Second, 6-Hydroxy-2-[2-(4'-methoxyphenyl) ethyl] chromone was detected in ethanol extract of the seed with 31.132 response.

Conclusion: Findings from the study tend to support the idea that the seed of E.zwageri can be utilized as effective bio-resources for designing novel health-promoting products or ingredients. Seed of E.zwageri is a resource for α and β Asarone, O-Methylviolanone, N-cis-Feruloyltypamine, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8-methoxy-chroman-4-one, and 6-Hydroxy-2-[2-(4'-methoxyphenyl)ethyl] chromone.