A novel open source tool for ELISA result analysis

ELISA has become a standard analytical tool in the numerous branches of science and industry. Processingof the ELISA results may be a multistep process, often requiring a prior adaptation, using proprietarysoftware, or exporting the results into external internet platforms. It may be problematic in the lightof good documentation practices and maintaining good data integrity. In this paper, we present thedevelopment and application of the ELISA Tool software. The program is based on a Python scriptingprogramming language and is available under an open-source license. The ELISA Tool allows users tofully control and validate the calculation procedure through a user-friendly graphical user interface.The modular architecture of the software allows its application in other information technology (IT)projects used for data processing in research laboratories. We successfully applied the ELISA Tool for theanalysis of real-life samples. The ELISA Tool allowed import of the measurement data, an approximationof the calibration curves with two different algorithms, exploration and diagnostics of the model fit,and generation of the final report with the calculations while maintaining the raw data file unchanged.We report here for the first time the implementation of the idea of full control over data processing,from measured raw data to the final report. We obtained a transparent, open, registered system of dataprocessing control, independent of third parties. The modular and flexible architecture of the createdsoftware encourages its further development following the individual demands of the users.