Published June 28, 2020 | Version v1
Dataset Open

PDX1-/NKX6.1+ progenitors derived from human pluripotent stem cells as a novel source of insulin-secreting cells

  • 1. HBKU / QBRI
  • 2. CMUQ
  • 3. HBKU / QCRI

Description

PDX1-/NKX6.1+ progenitors derived from hESCs were differentiated to pancreatic beta cell stage where they generated glucose-responsive, insulin-secreting cells. The transcriptome profile of hESC- derived PDX1-/NKX6.1+ progenitors were compared with hPSC-derived PDX1+/NKX6.1+ pancreatic progenitors as described in the article entitled "PDX1-/NKX6.1+progenitors derived from human pluripotent stem cells as a novel source of insulin-secreting cells" by Memon et al. 2020. Furthermore, beta cells derived from PDX1-/NKX6.1+ progenitors (3D-beta) were compared with their own endocrine progenitors at an earlier stage (stage 5, 3D-S5) to map their development. 

The file name is : Sample name_replicate_sample ID_read direction_001 where:

-3D-beta: insulin-expressing cells derived from self-detached, 3D, PDX1-/NKX6.1+ progenitors

-3D-S5: endocrine progenitors from stage 5, derived from self-detached, 3D, PDX1-/NKX6.1+ progenitors

- RNA-seq data were generated from two biological replicates in most cases.

- Read direction: R1 (Forward), R2 (Reverse).

Files

Files (33.7 GB)

Name Size Download all
md5:4da38f082d4aef698d7860d4d52837a7
3.2 GB Download
md5:9f790a49950998f83cf07ac2fe4facca
3.5 GB Download
md5:7d8526528afd32cb8cc8a811f66f9ddf
2.2 GB Download
md5:f9efdc538efe3f20ac5661422ab50f71
2.4 GB Download
md5:bb43a0e268c9c32206afb4502b0724a6
1.8 GB Download
md5:c3eafb976f96c6da3bac0f5f74130be1
2.0 GB Download
md5:f546848b21901ec086d30c02a1f331b2
1.9 GB Download
md5:04b9a5d7ae8918019946fedacf178e8f
2.1 GB Download
md5:e4440fe786ebaeae5ad654227b886861
2.3 GB Download
md5:f92199e0b9758de3b7f4f77e5ee784a9
2.8 GB Download
md5:33c687bd53153cff8758a9523db48d0a
4.1 GB Download
md5:0964ead6bf2eaca494c19a40fd86fd68
5.3 GB Download