THE CORRESPONDENCE OF POLYMORPHISM С677Т OF METHYLENE TETRA HYDROFOLATE REDUCTASE GENE WITH CARDIOVASCULAR DISEASES IN THE POPULATION OF AZERBAIJAN

OBJECTIVE: The research is devoted to the studying of the C677T polymorphism of the MTHFR gene among patients with cardiovascular diseases - coronary heart disease, myocardial infarction and hypertension

METHODS: We used a complex of molecular genetic methods including: polymerase chain reaction in different execution modes, electrophoresis of genomic DNA and amplified gene fragments on agarose plates and nucleotides of individual fragments to determine the type of mutation.

RESULTS: As a result of sequencing of a fragment of the MTHFR gene in 72 patients, in 27 cases (37.5%), the mutation C677T MTHFR (Ala677Val) in the heterozygous state (C / T) was identified. When comparing the obtained mutation values among people in the control and experimental groups, it was found that the frequency of occurrence of the C677T MTHFR mutation (Ala677Val) among the group of patients was 15.3% higher than that in people in the control group (p <0, 05).

CONCLUSION: As a result, a high frequency of these mutations was established within the group of patients with severe forms of cardiovascular disorders. For the first time there also were determined the frequencies of these mutations in the population of Azerbaijan and was postulated that these frequencies are mainly correlated with the frequencies described in other populations of the world.

Proper prophylaxis by detecting the C677T mutation of the MTHFR gene will allow doctors to conduct qualified treatment of cardiovascular diseases