Published June 22, 2020 | Version v1

Data from: "Rapid molecular evolution of Spiroplasma symbionts of Drosophila"

  • 1. Department of Medical and Biological Sciences, Oxford Brookes University, UK
  • 2. Department of Ecology and Conservation Biology, Texas A&M University, College Station, Texas, USA
  • 3. Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne, Switzerland
  • 4. Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, UK
  • 5. Department of Biology, Texas A&M University, College Station, Texas, USA

Description

This repository contains data and information to reproduce the findings reported in the paper.

File descriptions:

  • OTU_sequences.fasta – all Spiroplasma sequences that contained an OTU domain as predicted by PfamScan
  • OTU_alignments.fasta – alignment of OTU domains performed using Mafft
  • RIP_sequences.fasta – all Spiroplasma sequences that contained an RIP domain as predicted by PfamScan
  • RIP_alignments.fasta – alignment of RIP domains performed using the HMMER package
  • Spiroplasma_supermatrix.fasta – Fasta alignment of concatenated single copy Spiroplasma loci conserved across the investigated strains. Loci that showed signs of recombination were not included
  • Spiroplasma_partitions.txt – Lists the loci that make up the Spiroplasma supermatrix
  • Spiroplasma_partitioning.scheme.txt – Partitioning scheme employed in our Maximum Likelihood analysis of the supermatrix. This was the best fitting partitioning scheme as determined with IQ-TREE
  • Protocol_1.pdf – Chloroform–Ethanol protocol used for extracting Spiroplasma DNA for sHy-Tx

Files

Protocol_1.pdf

Files (937.6 kB)

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Additional details

Funding

European Commission
Symbcompat - Determing symbiont factors that affect compatibility with a novel host 703379