Published June 4, 2020
| Version V1.0
Technical note
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Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology
- 1. MNI, McGill University
Description
This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:
- CRISPR-Cas9 editing;
- Droplet digital PCR (ddPCR) technology;
- Single cell sorting.
Files
EDDU-013-01_CRISPR editing of non-iPSCs through ddPCR_v1.0.pdf
Files
(2.3 MB)
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