Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.

This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:

  • CRISPR-Cas9 editing;
  • Droplet digital PCR (ddPCR) technology;
  • Single cell sorting.

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EDDU-013-01_CRISPR editing of non-iPSCs through ddPCR_v1.0.pdf
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