Raw diffraction images of heliorhodopsin E108D mutant

Crystal structure of Thermoplasmatales archaeon heliorhodopsin E108D mutant (PDB code: 7CLJ).

79 small-wedge (10°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 5×5 µm² and oscillation step was 0.1°. The crystals belonged to space group P4₂2₁2 with unit cell parameters a~72.9, c~115 Å.

52 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 12 integrated results were merged at 2.6 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result (Tanaka et al. BBRC, 2020).