Implementation of Genomic Variant Calling Using GATK4, SPARK, WDL, CROMWELL and DOCKER Over Simulated Ebola NGS Dataset.

Ebola genome is manually mutated to contain structural variants. There are seven mutated ebola genomes each one for two large deletions, insertions, duplications, translocations, inversions, one complex variant1 (consecutive three mutations - insertion, duplication and deletion) and one complex variants2 (consecutive three mutations - deletion, duplication and deletion). All insertions are novel sequence insertion. Paired-end illumine RNASEQ reads are simulated in fastq format.