Published May 20, 2020 | Version v1

Cytosolic crowding drives the dynamics of both genome and cytosol in Escherichia coli challenged with sublethal antibiotic treatments

  • 1. University of Cambridge
  • 2. école Normale Supérieure
  • 3. Sorbonne Université

Description

Datasets used in the publication:
"Cytosolic crowding drives the dynamics of both genome and cytosol in Escherichia coli challenged with sublethal antibiotic treatments". 
The archives are named according to the treatment administered and contain microscopy images of the chromosomal locus ORI2.
21 fields of view were imaged for 45s at a 9.6 frame rate per second (producing 442 images) every 20 minutes for up to two hours. 
The number after "Scan" indicates the 20 minutes intervals (1 to 6 meaning 20 to 120 minutes). The number after "Position" indicates the field of view (1 to 21). Each of these folders contains 442 images. 

Every archive also contains phase contrast images, which are in the folders containing PhC (for Phase Contrast) in the name. Each of these folders contains 21 folders (one for each position) which in turn contain 6 images, taken at the beginning of the aforementioned 45s intervals.

Due to space limitations, we could only upload one experiment (out of 3 replicates) from only one of the 3 markers (Ori, Ter and cytoplasmic). The remaining data is available upon reasonable request from the corresponding author of the publication.

The Excel files contain data points values and raw measurements for the plots given in Fig. 2, 6A, 6B, 6D and SI11B&C and SI12.

The codes used to analyse the data are given at: https://github.com/ver228/bacteria-loci-tracker 

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