Technical note Open Access
Rocha, Cecilia; Tabatabaei, Mahdieh; Chen, Xiuqing; Lepine, Paula; Mathur, Meghna; Mohamed, Nguyen-Vi; Pimentel, Luisa; Durcan, Thomas M.
This protocol describes how to generate forebrain spheroids from iPSCs. Forebrain spheroids are formed in stationery cultures, they contain cortical neurons and astrocytes and can be maintained in cultures for several months allowing the study of neuronal progenitors and mature cells. Thus, Forebrain spheroids provide a 3D model to study cortical development and brain activity.
EDDU-015-01_Generation of Forebrain organoids_v1.0.pdf