Technical note Open Access

Generation and profiling of Forebrain Spheroids

Rocha, Cecilia; Tabatabaei, Mahdieh; Chen, Xiuqing; Lepine, Paula; Mathur, Meghna; Mohamed, Nguyen-Vi; Pimentel, Luisa; Durcan, Thomas M.

This protocol describes how to generate forebrain spheroids from iPSCs. Forebrain spheroids are formed in stationery cultures, they contain cortical neurons and astrocytes and can be maintained in cultures for several months allowing the study of neuronal progenitors and mature cells. Thus, Forebrain spheroids provide a 3D model to study cortical development and brain activity.

Files (691.2 kB)
Name Size
EDDU-015-01_Generation of Forebrain organoids_v1.0.pdf
691.2 kB Download
All versions This version
Views 162162
Downloads 7272
Data volume 49.8 MB49.8 MB
Unique views 155155
Unique downloads 6969


Cite as