GUV membrane linearization macro
Creators
- 1. Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany; International Max Planck Research School for Molecular Biology at the University of Göttingen, Germany
Description
GUV membrane linearization macro v1.0.0
http://dx.doi.org/10.5281/zenodo.376618
This package contains:
GUV_membrane_linearization_macro.ijm
This macro was developed by Agata Witkowska for the study
Witkowska A. & Jahn R., Biophysical Journal (2017)
"Rapid SNARE-mediated fusion of liposomes and chromaffin granules with giant unilamellar vesicles"
http://dx.doi.org/10.1016/j.bpj.2017.03.010
This Fiji macro can be used for automatic detection of GUV on a microscopy image (1 GUV on image present), linearization of its membrane with a polar transformation method, removal of saturated pixels and measurement of peak membrane intensities on a transformed image. It returns GUV diameter, mean membrane peak intensity and std of it.
Required plugin: Polar Transformer.
Files
GUV_membrane_linearization_macro.zip
Files
(2.2 kB)
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Additional details
Related works
- Is supplement to
- 10.1016/j.bpj.2017.03.010 (DOI)