Published April 8, 2020 | Version v1

Highly multiplexed histology reveals phenotypic and spatial characteristics of human Innate Lymphoid Cells in chronic inflammation - MELC tonsil data-set

Authors/Creators

  • 1. DRFZ and Immune Dynamics, Rheumatology an Clinical Immunology, Charité - Universitätsmedizin Berlin

Description

53 marker MELC Run in human tonsil. Each image depicts the same field of view, sequentially stained with the depicted fluorescence-labelled antibodies. Images contain 2048 x 2048 pixels and are generated using an inverted wide-field fluorescence microscope with a 20x objective, a lateral resolution of 325 nm and an axial resolution above 5 µm. Images have not been normalized and intensities have not been adjusted.

 

Notes

This study was supported by funding from the Deutsche Forschungsgemeinschaft, (SPP1937, HA5354/8-2 to A.E.H.) The work was supported by an "Adding 3R Value" grant of the Charité - Universitätsmedizin, Berlin.

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Additional details

Related works

Is source of
10.5281/zenodo.3744206 (DOI)
Is supplement to
10.5281/zenodo.3744173 (DOI)