Published February 24, 2020
| Version V1.0
Technical note
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CRISPR-mediated introduction of single nucleotide changes in iPSCs
Description
This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.
Files
EDDU-009-01_CRISPR editing of iPSCs_v1.0.pdf
Files
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