Technical note Open Access

CRISPR-mediated introduction of single nucleotide changes in iPSCs

Deneault, Eric; Nicouleau, Michael; Durcan, Thomas M.

This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.

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