Technical note Open Access

Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)

Del Cid Pellitero, Esther; Shlaifer, RIna; Luo, Wen; Krahn, Andrea; Nguyen-Renou, Emmanuelle; Manecka, Destiny-Love; Rao, Trisha; Beitel, Lenore; Durcan, Thomas M.

This protocol describes how to:
• Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
• Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)

Version 2.0 now available. 

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