Technical note Open Access
Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)
Del Cid Pellitero, Esther; Shlaifer, RIna; Luo, Wen; Krahn, Andrea; Nguyen-Renou, Emmanuelle; Manecka, Destiny-Love; Rao, Trisha; Beitel, Lenore; Durcan, Thomas M.
This protocol describes how to:
• Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
• Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)
Version 2.0 now available.
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(2.6 MB)
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EDDU-007-02_QC Characterization of a-syn PFFs_v2.0.pdf
md5:c84eb88626f4beca63aba01b71bb7d61 |
2.6 MB | Download |
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Indexed in
- Publication date:
- September 4, 2019
- DOI:
-
- Keyword(s):
- alpha-synuclein quality-control fluorescent imaging electron microscopy dynamic light scattering (DLS)
- Communities:
- License (for files):
- Creative Commons Attribution 4.0 International