Technical note Open Access

Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)

Del Cid Pellitero, Esther; Shlaifer, RIna; Luo, Wen; Krahn, Andrea; Nguyen-Renou, Emmanuelle; Manecka, Destiny-Love; Rao, Trisha; Beitel, Lenore; Durcan, Thomas M.

This protocol describes how to:
• Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
• Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)

Version 2.0 now available. 

Files (2.6 MB)
Name Size
EDDU-007-02_QC Characterization of a-syn PFFs_v2.0.pdf
md5:c84eb88626f4beca63aba01b71bb7d61
2.6 MB Download
377
269
views
downloads
All versions This version
Views 377152
Downloads 26984
Data volume 522.2 MB217.4 MB
Unique views 337140
Unique downloads 25070

Share

Cite as