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Published August 21, 2019 | Version V2.0
Technical note Open

Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Creators

  • 1. MNI, McGill University

Description

This protocol describes how to:
• Produce and purify recombinant human and mouse α-synuclein in monomeric form from transformed bacteria
• Generate α-synuclein preformed fibrils (PFFs) from recombinant monomeric α-synuclein
• Fluorescently label α-synuclein PFFs

Version 2.0 now available

Files

EDDU-006-02_Production of a-syn monomers and PFFs_v2.0.pdf

Files (3.0 MB)