SAUSSUREA OBVALLATA AND SAUSSUREA SIMPSONIANA A NEW SOURCE OF DEHYDROCOSTUS LACTONE (IMPORTANT ANTICANCER COMPOUND) FROM NORTHWEST HIMALAYA

HPTLC technique for analysis of Dehydrocostus- lactone developed and validated for their etermination of dehydrocostus- lactone, root and flower extracts of Saussurea simpsoniana, Saussurea obvallata. The analytes were extracted with 70% methanol and tested on TLC aluminum plates along with standard. Analysis was executed on pre-coated TLC aluminum plates. Linear ascending development was completed in twin trough glass chamber saturated with mobile phase comprising of toluene: ethyl acetate (9:1 v/v), Spectro- densitometric scanning was performed by TLC scanner IV (CAMAG) in absorbance mode at the wavelength of 530-nm. Present, study revealed that marker compounds are present in higher amount in the roots of S. obvallata, (12.19 mg/g), while the concentration was low 5mg/g in the root of S. simpsoniana extracts and was not detected in flower sample of S. simpsoniana.