Dataset related to article "CXCR3 Identifies Human Naive CD8+ T Cells with Enhanced Effector Differentiation Potential."
Authors/Creators
- De Simone G1
- Mazza EMC1
- Cassotta A2
- Davydov AN3
- Kuka M4
- Zanon V1
- De Paoli F1
- Scamardella E1
- Metsger M3
- Roberto A1
- Pilipow K1
- Colombo FS1
- Tenedini E5
- Tagliafico E5
- Gattinoni L6
- Mavilio D1
- Peano C1
- Price DA7
- Singh SP8
- Farber JM8
- Serra V9
- Cucca F9
- Ferrari F10
- Orrù V9
- Fiorillo E9
- Iannacone M5,4
- Chudakov DM11
- Sallusto F2
- Lugli E1
- 1. Humanitas Clinical and Research Center – IRCCS -, via Manzoni 56, 20089 Rozzano (Mi) - Italy
- 2. Institute for Research in Biomedicine, Faculty of Biomedical Sciences, USI, 6500 Bellinzona, Switzerland.
- 3. Central European Institute of Technology, 621 00 Brno, Czech Republic
- 4. Division of Immunology, Transplantation and Infectious Diseases and Experimental Imaging Center, IRCCS, San Raffaele Scientific Institute and Vita-Salute San Raffaele University, 20132 Milan, Italy.
- 5. Department of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy
- 6. Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892.
- 7. Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom.
- 8. Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
- 9. IRGB, National Research Council, 09042 Monserrato, Italy.
- 10. IFOM, FIRC Institute of Molecular Oncology, 20139 Milan, Italy.
- 11. Central European Institute of Technology, 621 00 Brno, Czech Republic.
Description
In mice, the ability of naive T (TN) cells to mount an effector response correlates with TCR sensitivity for self-derived Ags, which can be quantified indirectly by measuring surface expression levels of CD5. Equivalent findings have not been reported previously in humans. We identified two discrete subsets of human CD8+ TN cells, defined by the absence or presence of the chemokine receptor CXCR3. The more abundant CXCR3+ TN cell subset displayed an effector-like transcriptional profile and expressed TCRs with physicochemical characteristics indicative of enhanced interactions with peptide-HLA class I Ags. Moreover, CXCR3+ TN cells frequently produced IL-2 and TNF in response to nonspecific activation directly ex vivo and differentiated readily into Ag-specific effector cells in vitro. Comparative analyses further revealed that human CXCR3+ TN cells were transcriptionally equivalent to murine CXCR3+ TN cells, which expressed high levels of CD5. These findings provide support for the notion that effector differentiation is shaped by heterogeneity in the preimmune repertoire of human CD8+ T cells.
Files
CXCR3 identifies human naive CD8+ T cells with enhanced effector differentiation potential 130320.zip
Files
(515.2 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:5bcfe753396b46922f78b0424804b62b
|
515.2 kB | Preview Download |
Additional details
Related works
- Is supplement to
- 31740485 (PMID)
- 10.4049/jimmunol.1901072 (DOI)