COMPARATIVE ANALYSIS OF ENAMEL MINERAL PROFILE USING VARIOUS REMINERALIZING AGENTS WITH CONFOCAL LASER RAMAN SPECTROSCOPY - AN INVITROSTUDY

Aim: To evaluate quantitative changes in enamel mineral profile using CPP-ACP, ACP and Bio-active dentine with confocal laser raman spectroscopy. Materials and Methods: Totally, 25extracted intact pre-molar teeth were collected as specimens. Then the specimens were sectioned at CEJ .The sectioned teeth were evaluated for mineral content using Confocal Laser Raman Spectroscopy (Group ?I).The specimens were placed in demineralizing solution for 4 days and were evaluated for mineral content using Confocal Laser Raman Spectroscopy (Group II).The teeth were then divided into other three groups (6 teeth each) and treated with MI Varnish (Group III) , Enamel Pro Varnish (Group IV), and Bio-active Glass mixed with pro-flouride varnish (Group V) and then dipped in artificial sliva for 5 days, remaining 6 teeth were dipped only in artificial sliva (Group VI) following which the teeth surfaces were studied using Raman spectroscopy to assess the remineralization potential of the three dentifrices. The data were recorded and analyzed statistically. Results: Raman spectroscopic analysis revealed better remineralization with Bio-active Glass mixed with pro-flouride varnish, from the groups treated with the MI Varnishand the Enamel Pro Varnish Conclusion: It can be concluded that the demineralized samples of teeth treated with Bio-active Glass mixed with pro-flouride varnish, was most effective in remineralization of early enamel caries at surface level when analyzed using Raman spectroscopy.