TO EXPLORE ANTIMICROBIAL AND METAL MEDIATION PROPERTIES OF RHAMNOLIPID EXTRACTED FROM PSEUDOMONAS SPP. FROM METAL CONTAMINATED SOIL

The heavy metal contamination and disposal of waste frying oil, as well as resistance, occurs due to the hydrophobic nature of antibiotics is a universal issue. The challenge is to develop a method in which biological molecules produced by the low-cost substrate used to reduce metal-associated toxicity in metal-contaminated waste. Along with that, such biological molecules can use alone or synergistically to makes the microbial cell surface more susceptible to accumulate hydrophobic substance (antibiotic) into the cell for restoring resistance. For this purpose, biosurfactant producing bacteria were isolated from a metal-contaminated soil sample and identified as Pseudomonas spp..The result showed that extracellular glycolipid produced by metal isolate was Rhamnolipid. Maximum rhamnolipid production observed when the culture was incubated for 6-8 days in medium containing different amount of mannitol and peptone. Fried oil also acts as the best carbon source giving excellent rhamnolipid production. Moreover, extracted rhamnolipid found to be antimicrobial against gram-positive, gram-negative organisms and fungi as well. It also increases its microbicidal activity of ampicillin antibiotic when used synergistically with rhamnolipid. Different concentrations (60, 80?g/ml) of rhamnolipid were evaluated and compared for the efficiency of heavy metal [Cr (VI)] removal. The result showed that superior adsorbent having 80 ?g/ml concentration of rhamnolipid reduced 46%, 39%, 32%, 19% of 10ppm, 20ppm, 60ppm, 80ppm of hexavalent chromium respectively.