Published January 30, 2020 | Version v1

High MHC gene copy number maintains diversity despite homozygosity in a Critically Endangered single-island endemic bird, but no evidence of MHC-based mate choice

  • 1. Institute of Ecology and Evolution, University of Oregon, Eugene OR 97403-5289, USA & Department of Biology, Lund University, Ecology Building, 223 62 Lund, Sweden
  • 2. Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, United Kingdom & Fauna & Flora International, The David Attenborough Building, Pembroke Street, Cambridge CB2 3QZ, United Kingdom
  • 3. Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, United Kingdom
  • 4. Department of Biology, Lund University, Ecology Building, 223 62 Lund, Sweden

Description

Raw sequence data from two amplicon libraries of MHC class I exon 3 of Raso Lark Alauda razae, sequenced on an Illumina Miseq. The two different libraries (two different Illumina runs) are collected in separat tar archive (.tar). Within each of those are individual sequence reads as gzipped fastq files (.fastq.gz). Each sample has two files, one for read 1 (R1) and one for read 2 (R2), with file names structured as follows. Delimited by underscore (_) are:

  1. sample name as referred to in the data and paper (“RingNo” in the Supporting data table);
  2. formal ID (also referred to in data table, often corresponding to full ring number);
  3. Illumina sample number (i.e. based on the order that samples are listed in the sample sheet);
  4. Illumina lane number (static as L001, as Miseq instruments have a single lane on their flow cells);
  5. read number (R1 [forward] or R2 [reverse]);
  6. static identifier from Illumina (001).

Thus, the file 83304_TJ83304_S163_L001_R2_001.fastq.gz is the reverse (read 2) MHC class I exon 3 sequence of individual 83304 (ring number TJ83304).

Files

Files (981.9 MB)

Name Size
md5:0c3b4b37785917a19703cb3f255fd39c
78.3 MB Download
md5:2b5dc7733975007c67a6cb8d2b676b8d
903.6 MB Download

Additional details

Related works

Is cited by
Preprint: 10.1101/2020.02.03.932590 (DOI)

Funding

European Commission
Optimal-Immunity - Optimal diversity in immunity – to overcome pathogens and maximize fitness; moving from correlative associations to a more mechanistic understanding using wild songbirds. 679799