Raw diffraction images of Drosophila Piwi
Authors/Creators
- 1. Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Description
Crystal structure of Drosophila Piwi (PDB code: 6KR6).
28 mercury-bound data and 4 native data were included (see file_list.txt for details). Each dataset consists of 180° (except two 90° datasets) from single crystal and was collected using EIGER X 9M detector at a wavelength of 1 Å with helical data collection scheme using 15×10 μm beam on BL32XU, SPring-8.
All diffraction images were processed using DIALS 1.10.2 through KAMO pipeline, and merged using XSCALE from XDS package with kamo.multi_merge. The crystals belong to space group P212121 with a=62.1, b=115.6, c=119.9 Å. Finally 23 mercury-bound datasets were merged at 2.9 Å resolution in the published result (Yamaguchi et al. Nature Communications, 2020). Merging improved resolution and electron density of PAZ domain that was difficult to interpret with a single dataset.
Files
file_list.txt
Files
(147.2 GB)
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Additional details
Related works
- Is cited by
- Journal article: 10.1038/s41467-020-14687-1 (DOI)