GENETIC VARIATION IN A JATROPHA CURCAS JATROPHA INTEGERRIMA HYBRID
Creators
- 1. Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco A. C. Subsede Sureste. Tablaje Catastral 31264 Km 5.5 Carretera Sierra Papacal - Chuburna Puerto Parque Cientifico Tecnologico de Yucatan CP: 97302 Merida, Yucatan, Mexico
- 2. Universidad Autonoma de Yucatan, Facultad de Ingenieria Quimica, Periferico norte, Km. 33.5, Tablaje catastral 13615, Chuburna de Hidalgo Inn, 97203 Merida, Yucatan, Mexico
- 3. Campo Experimental Chetumal, INIFAP, Carretera Chetumal-Bacalar, Km 25, Chetumal, Quintana Roo, Mexico. C.P. 77900
- 4. Departamento de Biotecnologia y Bioquimica. Centro de Investigacion y de Estudios Avanzados del IPN, Unidad Irapuato. Irapuato, Guanajato., CP. 36821. Mexico
Description
A viable hybrid was produced by an interspecific Jatropha curcas x Jatropha integerrima cross. Artificial cross pollination was used, with J. integerrima being the pollen donor for J. curcas. Hybrid fruit resulted. Seeds from this fruit were germinated in vitro to produce seedlings as a source of leaf explants for genomic DNA extraction and purification. Molecular markers (ISSR and DAMD) were used to confirm hybridization success and characterize the combination of parental characteristics in the hybrid. Based on the band pattern, this interspecific cross resulted in genetic variation in the hybrids DNA, since 15% of its DNA content exhibited no similarity to either parent genome. Of the remaining DNA content, 35% was similar to the female parent (J. curcas), 25% to the male parent (J. integerrima), and 25% was a combination of both parents.
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