C. elegans HSP-17 translational reporter LSM z-stacks
Description
A translational reporter (transgene: phsp-17::hsp-17::wrmScarlet) of the small heat shock protein HSP-17 from C. elegans was generated. This construct expresses a C-terminal fusion of wrmScarlet to HSP-17 in a wild type background, controlled by the native promoter of hsp-17. A publication, for which this worm strain was generated, titled "The non-canonical small heat shock protein HSP-17 from C. elegans is a selective protein aggregase" to JBC is pending (manuscript number: JBC/2019/011185). The dataset contains the raw LSM-data, a version modified for easier viewing as well as a short movie of a 3d-projection. Data for the worms head, body and tail are given.
For wrmScarlet, refer Bindels et al., 2016 & El Mouridi et al., 2017. Data was evaluated and modified with Fiji (Rueden et al., 2017 & Schindelin et al., 2012).
Files
hsp-17 translational body z-stack.tif
Files
(529.2 MB)
| Name | Size | |
|---|---|---|
|
md5:af8867c82ac2788a1b5c81dbcfa79ef8
|
10.5 MB | Download |
|
md5:784b51fe244d788a078eda6d78b853ac
|
122.5 MB | Download |
|
md5:2aa6cc328035d649f72af432c0ba5399
|
33.9 MB | Preview Download |
|
md5:9909c7bdfb6a0eba0df735fb36e9e649
|
8.8 MB | Download |
|
md5:6ae7b0b6a4f555f57f850770fdacb401
|
152.5 MB | Download |
|
md5:e788a9c618ff8ee6295339625f6b4871
|
29.3 MB | Preview Download |
|
md5:9ab2bb8c0e6fa3fc6a590da9d78b789f
|
10.3 MB | Download |
|
md5:f779f96c5e8420b6e237628fb3289ae4
|
137.5 MB | Download |
|
md5:b773f13c73da4a96b4d0f0331835b475
|
23.9 MB | Preview Download |
Additional details
References
- Rueden, C. T., Schindelin, J., Hiner, M. C., DeZonia, B. E., Walter, A. E., Arena, E. T., and Eliceiri, K. W. (2017) ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics 18, 529
- Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J. Y., White, D. J., Hartenstein, V., Eliceiri, K., Tomancak, P., and Cardona, A. (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9, 676-682
- Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., and Gadella, T. W. J. (2016) mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods 14, 53-56
- El Mouridi, S., Lecroisey, C., Tardy, P., Mercier, M., Leclercq-Blondel, A., Zariohi, N., and Boulin, T. (2017) Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype. G3 (Bethesda) 7, 1429-1437