EVALUATION OF ANTIOXIDANT AND NEUROPROTECTIVE EFFECT OF POLY HERBAL COMPOUND AGAINST SCOPOLAMINE INDUCED MEMORY IMPAIRMENT IN ANIMAL MODEL

The present study was designed to evaluate the anti oxidant and neuroprotective effect of ethanolic extract of eleusine corocana, cyperus rotandus and moringa oleifera against scopolamine induced memory impairment in animal model. The study was carried for 21 days.The Alzheimer’s disease was induced using scopolamine 2 mg/kg b.w The phytochemical screening showed the presence of flavonoids, steroids, phenols, terpenoids, glycosides and alkaloid compound. Alzheimers disease was screened by observing behavioural parameters using actophotometer, elevated plus maze and biochemical estimations like acetyl cholinesterase, catalase, glutathione, lipid peroxidation and super oxide dismutase  The treatment with ethanolic extract of eleusine corocana,cyperus rotandus and moringa oleifera (EEECM) 200 mg/kg and 400 mg/kg effectively decreased the AChE and malondialdehyde levels when compared with the disease control group and increased the anti oxidant enzyme . Histopathological studies were performed where disease control group showed  the presence of neurofibrillary tangles and tau proteins accumulations  in the hippocampus and apoptic neurons and inflammation in the cerebral cortex.and the treated groups of  EEECM 200mg/kg and 400 mg/kg showed similar results to standard group where the cerebral cortex and hippocampus appeared normal and also showed mild proliferations in the region of hippocampus . Thus, the results suggest that EEECM possess anti oxidant and neuro protective properties.

Key words: Alzheimers disease, Scopolamine, Donepezil, Ethanolic extract, Eleusine corocana, Cyperus rotandus, Moringa oleifera, Acetyl cholinesterase.