Published January 4, 2013
| Version v1
Journal article
Open
Influence of Cytokinin and Auxin Types and Concentrations on In Vitro Shoot and Root Regeneration of Cactus Pear [(Opuntia ficus-indica (L.) Mill.]
Authors/Creators
- 1. Ambo University College of Agriculture and Veterinary Science, Department of Plant Sciences and Horticulture, P.O.Box 19, Ambo, Ethiopia
- 2. Haramaya University College of Agriculture and Environmental Sciences, Department Of Plant Sciences, P.O.Box 138, Dire-Dawa, Ethiopia
- 3. Tigray Agricultural Research Institute, P. O. Box 492, Mekelle, Tigray, Ethiopia
Description
An experiment was conducted with the aim of developing a protocol for the in vitro propagation of cactus pear from in vitro derived areole explants. The protocol involves subsequent in vitro morphogenesis and rooting of the in vitro proliferated shoots. For the shoot proliferation stage, explants each with 1 to 2 areoles were cultured on Murashige and Skoog medium (MS) supplemented with benzyladenine (BA) and kinetin (Kin) each with concentrations of 0, 0.5, 1.0, 2.0 and 4 mg/l and two combinations (0.5 mg/l BA + 4.0 mg/l Kin and 0.5 mg/l Kin + 4.0 mg/l BA). Analysis of the results indicated that there was no significant difference among the treatments used in terms of percentage of bud formation.