Published November 26, 2019
| Version v1
Dataset
Open
Diamond database for taxonomic annotation of fungal metatranscriptomics
Description
This is a protein fasta dataset for use with diamond. The fasta.gz file contains protein sequences for the following:
- 1,164 genomes downloaded from JGI (taxonomy_taxids.tsv)
- 121 genomes that are part of the taxmapper database (taxmapper_taxonomy_taxids.tsv) of which 6 were fungal
- the Hygrophorus russula MG78 genome downloaded from NCBI.
For the H. russula genome, genes were predicted using Augustus (v. 3.2.3) with the laccaria_bicolor model.
The final protein database consists of a total of 17,694,143 protein sequences (14,976,193 from JGI, 2,708,401 from taxmapper and 9,549 from H. russula).
The fasta file and associated taxonomic information files (nodes.dmp.gz & taxonmap.gz) can be used to build a diamond database compatible with diamond version 0.9.22:
zcat fasta.gz | diamond makedb -d diamond --taxonmap taxonmap.gz --taxonnodes nodes.dmp