Results of Study on Effect of Antischemin Preparation on Antioxidant System and Vascular Endothelial During the Cerebral Ischemia Model

Self-regulating mechanism and driving process of the organism has been a subject matter for the science for many years. Then, very simple determination of antioxidant system which is one of the above regulation is that it is unique biological system that keeps alive nature of the cell, origin for the humanity existence and studying changes and activity of the system on Antischemin is one set of the experiment. Research was carried out on ischemia model through methodology of Farкes E et al., 2007 with in the purpose to study effects of Antischemin on superoxidismutase, glutathione, glutathione peroxidase (GSH-Px pmol/ml), GABA /γ-aminobutiric acid/ and Vascular endothelial growth factor (VEGF) which indicate antioxidant activity. According to the results of the study glutathione (GSH-ng/L) level in cerebral tissue homogenate has decreased by 26-40.1%, superoxide dismutase (SOD) by 25.8-41%, glutathione peroxidase (GSH-px-pmol/ml) 15.4 by 44.5% and by 57.3-60.9% in the plasma respectively on 1^st to 21^st days after forming cerebral ischemia on trial animals and it proves rapid reduction of endogenous antioxidants in cerebral and cardiac ischemia. And in animals on which 100 mg/kg Antischemin was used, glutathione (GSH-ng/L) has increased by 16.1-27.2% in tissue homogenate, superoxide dismutase (SOD) by 14-20.9%, glutathione peroxidase by up to 14.3-31.1% on 1^st to 21sr trial days and it ensures condition to provide tissue with high energy substances such as ATP by increasing endogenous antioxidant reserve during oxygen deficiency. Also 16.7-20.95% decrease of VEGF-pg/ml in control group shows unstable metabolism in vascular and tissue causing main factor to damage vessel during cerebral hypoxia and among the group that used Antischemin VEGF-pg/ml has increased by 11.9-17.9% in brain homogenate and by 7.8-10.5% in comparative group, Bilobil, relatively and it indicates that substances influence during new vessel formation even not so strong.