Induction of matrix metalloproteinase'1 in in vitro experimental wound model using a novel three-dimensional culture system

The aim of this study was to determine whether experimentally punched wounds may influence the connective tissue metabolism of human fibroblast in a three dimensional culture supplemented with L-ascorbic acid. This culture was designed for human dermal
fibroblasts to organize a three-dimensional dermis-like structure by accumulating self-produced extracellular matrixes. In order to examine the effects of the wound, mRNA expression and production of type I collagen, matrix metalloproteinase-1 (MMP-1) and
tissue inhibitors of metalloproteinase-1 (TIMP-1) were sequentially examined up to 72 hrs after the wound was created.