ADVANTAGES OF CIRCULATION EXTRACTION FOR ISOLATION OF FLAVONOIDS FROM FLORES SOPHORAE JAPONICAE

Protunkevych O. O., Prysiazhniuk K. O., Rakipov I. M. ADVANTAGES OF CIRCULATION EXTRACTION FOR ISOLATION OF FLAVONOIDS FROM FLORES SOPHORAE JAPONICAE. Актуальні проблеми транспортної медицини / Actual problems of transport medicine / 2019;3(57):108-118. ISSN 1818-9385

DOI http://dx.doi.org/10.5281/zenodo.3465426

http://aptm.org.ua

УДК 66.048.5: 577.164.3: 661.12

ADVANTAGES OF CIRCULATION EXTRACTION FOR ISOLATION OF FLAVONOIDS FROM FLORES SOPHORAE JAPONICAE

Protunkevych O. O., Prysiazhniuk K. O., Rakipov I. M.

Odessa National Polytechnic University

Odessa, Shevchenko ave., 1, 65044

E-mail: o.o.protunkevych@opu.ua

Summary

Phyto-preparations based on extracts from plant raw materials have an important place in modern pharmacy. Medicinal plant Japanese pagoda tree (Sophora japonica), family Fabaceae, contains a rutin, which is a bioactive compound with pronounced antioxidant activity. Rutin drugs have capillary strengthening, anti-inflammatory and anti-edema effects. The raw material base expansion for rutin production is a priority of modern research in the pharmacognosy. It is of interest in-depth study of flavonoids content in Sophora japonica various organs and modern methods optimization of rutin extraction from raw materials. The study aim: circulation extraction optimal conditions determination for a flavonoids complex from Flores Sophorae japonicae, optimal concentration determination of ethyl alcohol for rutin isolation, determination of conditions for flavonoids recrystallization, and also the target product qualitative analysis. Dry Flores Sophorae japonicae crushed to 3-5 mm particles were used for the studies. The rutin was isolated by circulation extraction in a Soxhlet apparatus. 50 %, 70 % and 96 % ethyl alcohol were used to determine the optimum concentration of the extractant. The dynamics of the rutin release at different concentrations of the extractant were investigated. In the experimental extracts, the quantitative content of flavonoids (in terms of rutin) was evaluated by spectrophotometry by the level of aluminum chloride complexation. The extracts were concentrated on a rotary evaporator for crystallization of rutin. The flavonoid precipitates were released from the impurities by recrystallization and subjected to qualitative analysis by TLC, differential spectrophotometry, and mass spectroscopy. Ethanol 70 % exhibited the best flavonoid extraction properties, for complete raw material depletion, the circulation extraction duration was 2 hours 30 minutes. A solvent system of 96 % ethanol-water was used for recrystallization, in the purified flavonoid precipitate the proportion of impurities was about 4 %. In the qualitative analysis, the presence of rutin and quercetin in the experimental sediments was confirmed by TLC, differential spectrophotometry and mass spectrometry. The circulating extraction method is promising to obtain the rutin of Flores Sophorae japonicae, it allows to achieve complete depletion of raw materials and the extractant significant savings.

Keywords: japanese pagoda tree flowers, rutin, quercetin, circulating extraction.