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Expression test and purification of SETDB1 catalytic domain constructs' for structural studies by X-ray crystallography.

Beldar, Serap; Dong, Cheng; Lei, Ming; Li, Yanjun; Loppnau, Peter; Min, Jinrong

Experiment: Expression test and purification of SETDB1 catalytic domain constructs’ for structural studies by X-ray crystallography.

Aim: In the present section of this study, we focused on the development of efficient bacterial expression systems to produce large amounts of soluble SETDB1 catalytic domain for structural studies. This report involves a summary of expression test results of different fragments of SETDB1 and purification of various fusion proteins.

The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
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