Published September 26, 2019 | Version v1
Dataset Open

Raw data supporting metabarcoding unsorted kick-samples research

Authors/Creators

  • 1. National Museums Scotland
  • 2. University of Guelph
  • 3. Natural History Museum
  • 4. Albany Museum

Description

Raw data supporting: Metabarcoding unsorted kick-samples facilitates macroinvertebrate-based bioassessment with increased taxonomic resolution, while outperforming environmental DNA.

Two-step PCRs were performed on each sample replicate using the Qiagen Multiplex PCR Plus Kit.

The pool was loaded onto an Illumina MiSeq at 9pM, with 5% Phi-X, using a 600 cycle V3 kit with 300 bp paired end sequencing (index read steps skipped).

Also available in the NCBI SRA: https://www.ncbi.nlm.nih.gov/sra/PRJNA629361

Files

Files (6.7 GB)

Name Size
md5:a719f280fbd4caa3415de560fc546c05
3.2 GB Download
md5:69a0e9bd10594a8926b1cbce4cc437f9
3.5 GB Download