Lysozyme Diffraction Data from Beamline ID7B2 (FlexX), CHESS (broadband monochromatic X-rays at 1.5% bandpass)

This the raw diffraction data for a lysozyme crystal collected at beamline ID7B2 (FlexX), CHESS, Cornell University. ID7B2 utilizes a dual W/B4C multilayer monochromator, resulting in a high-flux beam with 1.5% bandpass. This sample dataset serves to demonstrate the utility of higher-bandwidth X-rays in protein crystallography.

These data can be readily treated as standard monochromatic diffraction data and can be processed with standard data processing programs such as XDS, iMosflm, and HKL2000.

Data processing parameters (NOTE: IMAGE HEADER METADATA IS UNRELIABLE)

• Detector: PILATUS3 6M
• Beam Center (pixels): X = 1260, Y = 1535
• Detector Distance: 414 mm
• Rotation Axis Phi: (XDS coordinates- "reverse phi" in iMosflm): -1 0 0
• Oscillation Range per frame: 0.1°
• X-ray wavelength: 0.9783 Å
• Temperature: 100 K