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Lesson Open Access

Training material for the mapping and quantification of single-cell RNA-seq 10X Datasets with RNA STAR Solo.

Tekman, Mehmet

The data provided here is part of the Galaxy Training Network tutorial that analyses 10x genomics single-cell RNA-seq data (scRNA) using cell barcodes and data from the 10x platform. This dataset of 1000 PBMC's from 10x

Due to time constraints during training, the datasets were subsampled in a method outlined in the subsetting_data.txt file.

The 10x Genomics Datasets follow the Creative Commons Attribution license, and the whitelist barcodes given in the CellRanger pipeline are distributed under the MIT license.

Files (6.9 GB)
Name Size
3M-february-2018.txt.gz
md5:eac3cdd869867e9e979d5da300a81823
18.4 MB Download
737K-august-2016.txt
md5:f62a276e262fdd85262a889d0f48556b
12.5 MB Download
cellranger_mit_license.txt
md5:9a7c338387b3e44dc84167099e2cea59
1.2 kB Download
Homo_sapiens.GRCh37.75.gtf
md5:1de18dd9077e0516b0ee756b0527f694
856.4 MB Download
pbmc_1k_v3_S1_L001_I1_001.fastq.gz
md5:da50f219b3ea514107a1901169916efd
255.9 MB Download
pbmc_1k_v3_S1_L001_R1_001.fastq.gz
md5:99efcdb237fa13cc7f92e1d91718c692
753.9 MB Download
pbmc_1k_v3_S1_L001_R2_001.fastq.gz
md5:e5cda97c012cfca47a43afdeb8e441ed
1.8 GB Download
pbmc_1k_v3_S1_L002_I1_001.fastq.gz
md5:17c48ffefc1582afeac23f06f6ad21f3
254.5 MB Download
pbmc_1k_v3_S1_L002_R1_001.fastq.gz
md5:e2300973dd787cc0a29b036490978fa8
748.7 MB Download
pbmc_1k_v3_S1_L002_R2_001.fastq.gz
md5:6b1e9d197dcf169d80d29b59c7254dbe
1.8 GB Download
subset_pbmc_1k_v3_S1_L001_R1_001.fastq.gz
md5:7b1eb908855832943c9dea36997c025d
66.9 MB Download
subset_pbmc_1k_v3_S1_L001_R2_001.fastq.gz
md5:e841faf4b9d57eb63aad284b97353e40
168.3 MB Download
subset_pbmc_1k_v3_S1_L002_R1_001.fastq.gz
md5:011a9d5926be3c143ea8916d70072245
74.9 MB Download
subset_pbmc_1k_v3_S1_L002_R2_001.fastq.gz
md5:1ab1853b1f068ace8a7301b0c96a81a0
187.5 MB Download
subsetting_data.txt
md5:54a06f258abe1751a215bb7c83fa7da0
4.9 kB Download
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