Lesson Open Access

Training material for the mapping and quantification of single-cell RNA-seq 10X Datasets with RNA STAR Solo.

Tekman, Mehmet

The data provided here is part of the Galaxy Training Network tutorial that analyses 10x genomics single-cell RNA-seq data (scRNA) using cell barcodes and data from the 10x platform. This dataset of 1000 PBMC's from 10x

Due to time constraints during training, the datasets were subsampled in a method outlined in the subsetting_data.txt file.

The 10x Genomics Datasets follow the Creative Commons Attribution license, and the whitelist barcodes given in the CellRanger pipeline are distributed under the MIT license.

Files (6.9 GB)
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3M-february-2018.txt.gz
md5:eac3cdd869867e9e979d5da300a81823
18.4 MB Download
737K-august-2016.txt
md5:f62a276e262fdd85262a889d0f48556b
12.5 MB Download
cellranger_mit_license.txt
md5:9a7c338387b3e44dc84167099e2cea59
1.2 kB Download
Homo_sapiens.GRCh37.75.gtf
md5:1de18dd9077e0516b0ee756b0527f694
856.4 MB Download
pbmc_1k_v3_S1_L001_I1_001.fastq.gz
md5:da50f219b3ea514107a1901169916efd
255.9 MB Download
pbmc_1k_v3_S1_L001_R1_001.fastq.gz
md5:99efcdb237fa13cc7f92e1d91718c692
753.9 MB Download
pbmc_1k_v3_S1_L001_R2_001.fastq.gz
md5:e5cda97c012cfca47a43afdeb8e441ed
1.8 GB Download
pbmc_1k_v3_S1_L002_I1_001.fastq.gz
md5:17c48ffefc1582afeac23f06f6ad21f3
254.5 MB Download
pbmc_1k_v3_S1_L002_R1_001.fastq.gz
md5:e2300973dd787cc0a29b036490978fa8
748.7 MB Download
pbmc_1k_v3_S1_L002_R2_001.fastq.gz
md5:6b1e9d197dcf169d80d29b59c7254dbe
1.8 GB Download
subset_pbmc_1k_v3_S1_L001_R1_001.fastq.gz
md5:7b1eb908855832943c9dea36997c025d
66.9 MB Download
subset_pbmc_1k_v3_S1_L001_R2_001.fastq.gz
md5:e841faf4b9d57eb63aad284b97353e40
168.3 MB Download
subset_pbmc_1k_v3_S1_L002_R1_001.fastq.gz
md5:011a9d5926be3c143ea8916d70072245
74.9 MB Download
subset_pbmc_1k_v3_S1_L002_R2_001.fastq.gz
md5:1ab1853b1f068ace8a7301b0c96a81a0
187.5 MB Download
subsetting_data.txt
md5:54a06f258abe1751a215bb7c83fa7da0
4.9 kB Download
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