Dataset Open Access

Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay

Mann, Mandeep; Harding, Rachel; Schapira, Matthieu

Expression and purification of biotinylated full length (FL) USP51-835 and biotinylated zinc finger ubiquitin-binding domain (ZnF-UBD) USP5171-290 constructs for the development of a displacement assay. 

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
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1_Purification and displacement assay experiment.pdf
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cloning.xlsm
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purification.pptx
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