Evaluating the effect of 8 M4K compounds on the viability of DIPG patient-derived cells (SU-DIPG-VI, HSJD-DIPG-007 and SU-DIPG-IV)

Viability assay using Celltiter Glo kit has been chosen as the main method for evaluating the efficacy of ALK2 inhibitors on DIPG patient-derived cells due to its robustness. As a pilot experiment, 8 ALK2 inhibitors with differing potencies were chosen and evaluated in 3 DIPG patient-derived cell lines (one with wild-type ALK2 and two with mutant ALK2).