COMPARISON OF NITRIC OXIDE AND MDA LEVELS IN TRAINED AND UNTRAINED INDIVIDUALS AT REST AND AFTER ACUTE MODERATE EXERCISE.

Introduction: Exercise is a stress like condition resulting in increase of reactive oxygen species (ROS), but if done regularly strengthens the antioxidant system.During the past few decades, information about the biological implications of exercise-induced oxidative stress has expanded rapidly. Aims And Objectives: No studies are available in literature to comment upon the levels of NO and MDA after acute moderate exercise. Therefore, we planned this study to compare the levels of NO and MDA in trained athletes and untrained healthy controls at rest and after 30 minutes of acute moderate exercise. Materials And Methods: Study was conducted on fifty athletes who do regular sports activities and are healthy. Blood was collected before and immediately after acute exercise for estimation of NO and MDA by Griess reaction and Thiobarbituric acid reactive species (TBARS) method respectively. Data was analyzed by student?s paired t test. Results And Observations:Research findings indicate that there were statistically significant differences in the levels of TG, TC, LDL-C, HDL-C, NO and MDA post-exercise as compared to the pre-exercise levels in athletic group (p= 0.02, 0.03, 0.03, 0.02, 0.005, and 0.03, respectively). There was no significant change in the levels of these variables in the control group before and after exercise. Pre-exercise values of TG, TC, LDL-C, HDL-C and MDA in trained athletes were lower than pre-exercise values in untrained group and the difference was significant statistically (p=0.03, 0.02, 0.04, 0.02 and 0.03 respectively). Conclusion: Acute moderate exercise in untrained individuals produces increased oxidative stress but after endurance training it has beneficial effects.