Published August 7, 2019 | Version v1
Dataset Open

EWAS results "Prediagnostic breast milk DNA methylation alterations in women who develop breast cancer"

Authors/Creators

  • 1. Geisel School of Medicine at Dartmouth
  • 2. University of Massachusetts Amherst
  • 3. University of South Carolina

Description

Prior candidate gene studies have shown tumor suppressor DNA methylation in breast milk related with history of breast biopsy, an established risk factor for breast cancer. To further establish the utility of breast milk as a tissue-specific biospecimen for investigations of breast carcinogenesis we measured genome-wide DNA methylation in breast milk from women with and without a diagnosis of breast cancer in two independent cohorts.

DNA methylation was assessed using Illumina HumanMethylation450k in 87 breast milk samples. Through an Epigenome Wide Association Study we explored CpG sites associated with a breast cancer diagnosis in the prospectively collected milk samples from the breast that would develop cancer compared with women without a diagnosis of breast cancer using linear mixed effects models adjusted for history of breast biopsy, age, RefFreeCellMix cell estimates, time of delivery, array chip, and subject as random effect.

The full analyses results are deposited here.

Notes

Table S1: EWAS results contralateral breast milk new cancer diagnosis after milk donation. Table S2: EWAS results ipsilateral breast milk new breast cancer diagnosis after milk donation. Table S3: EWAS results contralateral breast milk previous breast cancer. Table S4: EWAS results ipsilateral breast milk previous breast cancer. This work was supported by funds of the National Institutes of General Medical Sciences [Centers of Biomedical Research Excellence (COBRE) Center for Molecular Epidemiology at Dartmouth P20GM104416 to M.R.K. and B.C.C.]; the National Cancer Institute [R01CA216265 to B.C.C., R01CA230478-01A1 to K.F.A.]; the National Institute of Environmental Health Sciences [P01ES022832 to M.R.K.]; and the Environmental Protection Agency [RD-83544201-1 to M.R.K.].

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Is cited by
Journal article: 10.1093/hmg/ddz301 (DOI)