Raw diffraction images of heliorhodopsin

Crystal structure of heliorhodopsin from Thermoplasmatales archaeon (PDB code: 6IS6).

Small-wedge (10-15°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm² and oscillation step was 0.1°. The crystals belonged to space group C222₁ with unit cell parameters a~52, b~110, c~108 Å.

In total 212 small-wedge and two helical (180 & 120°) datasets were collected, and of these 179 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 107 integrated results were merged at 2.4 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result (Shihoya et al. Nature, 2019).

This upload also includes SeMet-labeled heliorhodopsin data (not actually used for phasing) and a different crystal form (tetragonal). SeMet data could be merged in P2₁2₁2₁ space group at 3.8 Å resolution. The tetragonal data are at 2.8 Å resolution.