Interaction of hypertensive rat serum immunoglobulin with recombinant human proteins

Microsoft Excel file: “HuProt supplemental data.xlsx”

Description of supplementary material : HuProt array v3.1 IgM and IgG signals from rat serum pools.

Sheet 1: “Array Z scores” Z scores for all array features for all sample pools used in the study detected with anti-IgM and anti-IgG reporter antibodies

Sheet 2: “IgM Z scores top 35” Z scores for the 35 array features that had highest scores detected with anti-IgM reporter in 28 week old salt loaded SHR-A3 and the corresponding scores for 16 week old SHR-A3 and 28 week old SHR-A3(IgH-B2) congenics.

Sheet 3: “IgM Z scores higher at 28 wks” Refinement of Sheet 2 to eliminate array features in which IgM Z scores were greater in 16 week old SHR-A3 prior to salt loading compared with 28 week old salt-loaded SHR-A3.

Sheet 4: “IgM Z scores congenics down” Refinement of Sheet 3 to include only array features in which IgM Z scores were reduced in 28 week old salt-loaded SHR-A3(IgH-B2) compared with 28 week old salt-loaded SHR-A3.

Sheet 5: “IgG Z scores top 35” Z scores for the 35 array features that had highest scores detected with anti-IgG reporter in 28 week old salt loaded SHR-A3 and the corresponding scores for 16 week old SHR-A3 and 28 week old SHR-A3(IgH-B2) congenics.

Sheet 6: “IgG Z scores higher at 28 wks” Refinement of Sheet 2 to eliminate array features in which IgG Z scores were greater in 16 week old SHR-A3 prior to salt loading compared with 28 week old salt-loaded SHR-A3.

Sheet 7: “IgG Z scores congenics down” Refinement of Sheet 3 to include only array features in which IgG Z scores were reduced in 28 week old salt-loaded SHR-A3(IgH-B2) compared with 28 week old salt-loaded SHR-A3.

Sheet 8: “Table 1 IgM IgG concordance” This data is presented as Table 1 in the manuscript and identifies those array features that are common to both Sheet 4 and Sheet 7, representing array features for which both IgM and IgG reactivity were reduced in 28 week old salt-loaded SHR-A3(IgH-B2) compared with 28 week old salt-loaded SHR-A3.