POTENCY OF PURIFICATION EXTRACT FROM BELIMBING WULUH (AVERRHOA BILIMBI) AS ANTIOXIDANT AND ANTI-TYROSINASE
Authors/Creators
- 1. Sekolah Tinggi Ilmu Farmasi "Yayasan Pharmasi Semarang" Jl. Letjend sarwo edie wibowo Km 1 Plamongansari Semarang, INDONESIA.
Description
ABSTRACT
Purpose: The purpose was to investigate purified extract of belimbing wuluh (PEBW) leaves as Indonesian plant for natural whitening agent.
Method: The purified extract of belimbing wuluh leaves used as a test Indonesian material. To investigate total phenolic content using folin-ciochalteu reagent, total flavonoids using AlCl3 reagent. The absorbance of belimbing wuluh were obtained on range 290-320 nm for SPF, 290-315 nm for percent erithema (%TE) 320-370 nm for percent pigmentation (%TP). Test for antioxidant activity with DPPH. Tyrosinase inhibitor activity using mushroom tyrosinase assay.
Results: Purified extract of belimbing wuluh leaves have a total phenolic content of 42.22±0.44 mgGAE/g and total flavonoid content of 41.38±1.31 mgRE/g. SPF values at a concentration of 300 ppm were obtained 10.64±0.66 with %TE 38.79±6.01% and %TP 9.28±0.79%. The IC50 value of antioxidant activity is 77.09±8.21 mg/mL greater than rutin 5.92 mg/ml. The IC50 values of Tyrosinase inhibitor activity is 224.16±34.55 mg/mL greater than kojic acid 16.68 μg/ml.
Conclusion: Purified extract of belimbing wuluh leaves have potential to be developed as sunscreen cosmetics and whitening agents.
KEYWORDS: Belimbing wuluh, Purified extract, Sunscreens, Antoxidant, Tyrosinase inhibitor.