Published May 2, 2019 | Version v1
Dataset Open

Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1 2019/05/02

Authors/Creators

  • 1. Structural Genomics Consortium, University of Toronto

Description

Project: Biophysical investigation of purified HTT protein and protein complex samples

Experiment: Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1

Date completed:­ 2019/05/02

Rationale: HAP1 and NEDD4L have been suggested as high probability interaction partners of HTT although it is unclear if they interact with HTT or HTT-HAP40. Coexpression of the constructs was conducted in baculoviral expression system (Sf9 cells) to determine if they copurify with HTT or HTT-HAP40. 

Notes

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

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Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1 20190502.pdf

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