The FluPRINT database

What is the FluPRINT database?

The FluPRINT represents fully integrated and normalized immunology measurements from eight clinical studies taken from 740 individuals undergoing influenza vaccination with inactivated or live attenuated seasonal influenza vaccines from 2007 to 2015 at the Stanford Human Immune Monitoring Center.

The FluPRINT dataset contains information on more than 3,000 parameters measured using mass cytometry, flow cytometry, phosphorylation-specific cytometry, multiplex cytokine assays, clinical lab tests (hormones and complete blood count), serological profiling and virological tests. In the dataset, vaccine protection is measured using a hemagglutination inhibition (HAI) assay, and following FDA guidelines individuals are marked as high or low responders depending on the HAI antibody titers after vaccination.

Want to know more?

To understand how the FluPRINT dataset was generated and validated, and how to use it, please refer to our open-access paper published in Scientific Data journal:

Tomic, A., Tomic, I., Dekker, C.L. et al. The FluPRINT dataset, a multidimensional analysis of the influenza vaccine imprint on the immune system. Sci Data 6, 214 (2019). https://doi.org/10.1038/s41597-019-0213-4

For additional exploration, please check out the project’s website: www.fluprint.com, where you can also explore the FluPRINT dataset on the following link: https://fluprint.com/#/database-access.

If you want to host your own FluPRINT database, please follow our instructions provided on the Github repository: https://github.com/LogIN-/fluprint.

How to use FluPRINT?

Here, you can download the entire FluPRINT database export as an SQL file, or as a CSV file. Additionally, we included the file with the SQL query to obtain those files.

Files are provided in two formats: zip and 7zip (7z). 7zip is a free and open-source file archiver available for download here: https://www.7-zip.org.

In the FluPRINT database, there are 4 tables: donor, donor_visits, experimental_data, and medical_history.
The exact description of each table is available in the FluPRINT paper.

Briefly, in the table donor, each row represents an individual with information about the clinical study in which an individual was enrolled (study ID and study internal ID), gender, and race. The second table, named donor_visits describes information about the donor’s age, cytomegalovirus (CMV) and Epstein-Barr virus (EBV) status, Body Mass Index (BMI), and vaccine received on each clinical visit. Information about vaccine outcome is available as geometric mean titers (geo_mean), the difference in the geometric mean titers before and after vaccination (delta_geo_mean), and the difference for each vaccine strain (delta_single). In the last field, each individual is classified as a high and low responder (vaccine_resp). On each visit, samples were analyzed and information about which assays were performed (assay field) and value of the measured analytes (units and data) are stored in the experimental_data table. Finally, the medical_history table describes information connected with each clinical visit about the usage of statins (statin_use) and if influenza vaccine was received in the past (influenza vaccine history), if yes, how many times (total_vaccines_received). Also, we provide information on which type of influenza vaccine was received in the previous years (1 to 5 years prior to enrolment in the clinical study). Lastly, information about influenza infection history and influenza-related hospitalization is provided.

How to cite FluPRINT?

If you use FluPRINT in an academic publication, please use the following citation: 

Tomic, A., Tomic, I., Dekker, C.L. et al. The FluPRINT dataset, a multidimensional analysis of the influenza vaccine imprint on the immune system. Sci Data 6, 214 (2019). https://doi.org/10.1038/s41597-019-0213-4

Contact Information

If you are interested to find out more about the FluPRINT, or if you experience any problems with downloading files, please contact us at info@adrianatomic.com.