Journal article Open Access

FORMULTION OF LIPOSOMES CONTAINING EXTRACT OF CYNODON DACTOLON Linn. BY 32 FACTORIAL DESIGN

Yogesh S. Thorat1, Naushad N. Mirza1*, Parikshit D. Shirure1, Avinash H. Hosmani2

Objective: The objective of the present study was to formulate and evaluate liposomes loaded with extract of Cynodon dactylon linn. Methods: Liposomes containing extract of Cynodon dactylon linn were made by thin layer film hydration method. Soya lecithin and cholesterol were used to make multiliamellar vesicles. Nine batches of liposomes were prepared based on the different weight ratio of Soya lecithin and cholesterol. Scanning Camera microscopy, IR, Drug entrapment, Dug release, Diffusion study, Stability study were conducted. Results: The prepared liposomes were evaluated by particle size analysis, entrapment efficiency, release study and stability study. Particle sizes were determined from the scanning Camera microscopy (SCM) photographs. It showed that F2, F5 and F8 batches had a good release results and smaller liposomes while F1, F3, F4, F6, F7 and F9 shows a poor release result with a mean at 269 µm. The percentage entrapment efficiency was found to be 76.13, 83.78, 73.44, 79.81, 86.19, 81.43, 74.32, 83.79, and 71.54 % respectively. The satisfactory batches F2, F5, and F8 was packed in containers and stored at 4°C temperature for one month. At the end of one month, the samples were analyzed for their physical properties, drug entrapment and in vitro release profile. The percentage release was found to be 77.4, 79.24, and 74.9% after 5 hrs. Conclusion: The formulation of Liposomes containing extract of Cynodon dactylon was carried out. The batches F1 to F9 were evaluated for Scanning camera microscopy, drug entrapment, drug release, diffusion study and stability study, the results are shows, The F2, F5, and F8 batches showed most promising results compared to other batches. No significant change was found during one month’s stability study of final batches.

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