Published May 8, 2019
| Version 1.0.0
Dataset
Open
Beta-lactam polyspecificity in E. coli
Authors/Creators
- 1. Novartis Institutes for BioMedical Research, Inc.
- 2. Novartis Institutes for BioMedical Research
Description
This image dataset supports the findings in our manuscript entitled Morphological deconvolution of beta-lactam polyspecificity in E. coli.
The images display fluorescently-labeled E. coli strains subjected to compound treatment at multiple concentrations. Cells were imaged in 96-well plates at 100X magnification. Our novel method for preparing E. coli cells for imaging enabled us to use commercially-available plate-based, automated HCI microscopes. We used the images to build our deep neural network classifier to identify bacterial morphologies as well as our Hidden Markov models for target deconvolution.